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LMO2 Antibody (PD00-25)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32542

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32542

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PD00-25

Concentration

1 mg/ml

Product Summary for LMO2 Antibody (PD00-25)

Immunogen

Recombinant protein full length human LMO2. (Uniprot: P25791)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LMO2 Antibody (PD00-25)

LMO2 Antibody (PD00-25)

Western Blot: LMO2 Antibody (PD00-25) [NBP3-32542]

Western Blot: LMO2 Antibody (PD00-25) [NBP3-32542] - Western blot analysis of LMO2 on K562 cell lysates with Rabbit anti-LMO2 antibody (NBP3-32542) at 1/1,000 dilution.

Lysates/proteins at 10 ug/Lane.

Predicted band size: 18 kDa
Observed band size: 18 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32542) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
LMO2 Antibody (PD00-25)

Immunohistochemistry: LMO2 Antibody (PD00-25) [NBP3-32542]

Immunohistochemistry: LMO2 Antibody (PD00-25) [NBP3-32542] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-LMO2 antibody (NBP3-32542) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32542) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for LMO2 Antibody (PD00-25)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:2000

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LMO2

LMO2 is a highly conserved oncoprotein that is expressed in human normal germinal-center B cells and in B-cell lymphomas. LMO2 also has a crucial role in hematopoietic development by being necessary for yolk sac erythropoiesis. LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number of T-cell acute lymphoblastic leukemia-specific translocations occur.

LMO2 antibodies are useful tools for erythropoiesis research and certian cancer studies.

Long Name

LIM Domain Only 2

Alternate Names

RBTN2, RBTNL1, RHOM2, TTG2

Gene Symbol

LMO2

Additional LMO2 Products

Product Documents for LMO2 Antibody (PD00-25)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LMO2 Antibody (PD00-25)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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