LMO4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43538

Novus Biologicals, part of Bio-Techne
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NBP2-43538
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Chicken (98%), Mouse (100%), Porcine (100%), Rat (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LMO4 Antibodies »

Product Specifications

Immunogen

Full length human LMO4 Recombinant protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LMO4 Antibody

Western Blot: LMO4 Antibody [NBP2-43538]

Western Blot: LMO4 Antibody [NBP2-43538]

Western Blot: LMO4 Antibody [NBP2-43538] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug whole cell extract of human LMO4-transfected 293T cells 12 % SDS-PAGE LMO4 antibody dilution: 1:5000.
Immunohistochemistry-Paraffin: LMO4 Antibody [NBP2-43538]

Immunohistochemistry-Paraffin: LMO4 Antibody [NBP2-43538]

Immunohistochemistry-Paraffin: LMO4 Antibody [NBP2-43538] - Analysis of human breast carcinoma. LMO4 antibody diluted at 1:500.
LMO4 Antibody

Western Blot: LMO4 Antibody [NBP2-43538] -

Western Blot: LMO4 Antibody [NBP2-43538] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with LMO4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for LMO4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LMO4

The LIM-only (LMO) proteins, LMO1 and LMO2, are nuclear factors that are characterized by a conserved LIM domain. The LIM domain consists of a cysteine-rich zinc-binding motif that is present in a variety of transcription factors, including the LIM homeobox (LHX) proteins expressed in the central nervous system and involved in cell differentiation. LMO1 and LMO2 are expressed in the adult CNS in a cell type-specific manner, where they are differentially regulated by neuronal activity and are involved in regulating the cellular differentiated phenotype of neurons. LMO2 lacks a specific DNA-binding homeobox domain but rather assembles into transcriptional regulatory complexes to mediate gene expression by interacting with the widely expressed nuclear LIM interactor (NLI). NLI, known also as CLIM-1, and the related protein CLIM-2 facilitate the formation of heteromeric LIM complexes and also enhance the nuclear retention of LIM proteins. LMO2 and the related protein LMO4 are expressed in thymic precursor cells. LMO4 is also expressed in mature T cells, cranial neural crest cells, somite, dorsal limb bud mesenchyme, motor neurons, and Schwann cell progenitors.

Long Name

LIM Domain Only 4

Alternate Names

Crp3, Etohi4

Gene Symbol

LMO4

Additional LMO4 Products

Product Documents for LMO4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LMO4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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