LMP2/PSMB9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52090

Novus Biologicals, part of Bio-Techne
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NBP1-52090
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rabbit (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all LMP2/PSMB9 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RNISYKYRED corresponding to internal region according to NP_002791.1.

Epitope

C-RNISYKYRED

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LMP2/PSMB9 Antibody

Western Blot: LMP2/PSMB9 Antibody [NBP1-52090]

Western Blot: LMP2/PSMB9 Antibody [NBP1-52090]

Western Blot: LMP2/PSMB9 Antibody [NBP1-52090] - Analysis of Proteasome 20S LMP2 in Human Thymus lysate (35ug protein in RIPA buffer) using NBP1-52090 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for LMP2/PSMB9 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 22 kDa band observed in human thymus lysates and approx. 20 kDa band in rat thymus (calculated MW of 23.3 kDa band according to human NP_002791.1 and rat NP_036840.2).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LMP2/PSMB9

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding different isoforms have been identified; both isoforms are processed to yield the same mature subunit.

Long Name

Proteasome (Prosome, Macropain) Subunit, beta Type, 9

Alternate Names

Proteasome subunit beta-1i, PSMB9, RING12

Entrez Gene IDs

5698 (Human); 16912 (Mouse); 24967 (Rat)

Gene Symbol

PSMB9

UniProt

NP_002791.1 (Human)

Additional LMP2/PSMB9 Products

Product Documents for LMP2/PSMB9 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LMP2/PSMB9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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