LMP7/PSMB8 Antibody (1B3)

Novus Biologicals, part of Bio-Techne | Catalog # H00005696-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1B3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LMP7/PSMB8 Antibodies »

Product Specifications

Immunogen

PSMB8 (AAH01114, 173 a.a. ~ 272 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DKKGPGLYYVDEHGTRLSGNMFSTGSGNTYAYGVMDSGYRPNLSPEEAYDLGRRAIAYATHRDSYSGGVVNMYHMKEDGWVKVESTDVSDLLHQYREANQ

Specificity

PSMB8 - proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional protease 7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for LMP7/PSMB8 Antibody (1B3)

Western Blot: LMP7/PSMB8 Antibody (1B3) [H00005696-M01]

Western Blot: LMP7/PSMB8 Antibody (1B3) [H00005696-M01]

Western Blot: LMP7/PSMB8 Antibody (1B3) [H00005696-M01] - Analysis of PSMB8 expression in transfected 293T cell line by PSMB8 monoclonal antibody (M01), clone 1B3.Lane 1: PSMB8 transfected lysate(29.8 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: LMP7/PSMB8 Antibody (1B3) [H00005696-M01]

Immunocytochemistry/ Immunofluorescence: LMP7/PSMB8 Antibody (1B3) [H00005696-M01]

Immunocytochemistry/Immunofluorescence: LMP7/PSMB8 Antibody (1B3) [H00005696-M01] - Analysis of monoclonal antibody to PSMB8 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody (1B3) [H00005696-M01]

Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody (1B3) [H00005696-M01]

Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody (1B3) [H00005696-M01] - Analysis of monoclonal antibody to PSMB8 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
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Applications for LMP7/PSMB8 Antibody (1B3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:1000

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has also been used for IF, IHC-P and ELISA. Use in Immunohistochemistry reported in scientific literature (PMID: 27213585).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LMP7/PSMB8

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.

Long Name

Large Multifunctional Protease 7

Alternate Names

ALDD, D6S216E, JMP, NKJO, Proteasome Component C13, Proteasome Subunit Y2, PSMB5i, PSMB8, RING10

Entrez Gene IDs

5696 (Human)

Gene Symbol

PSMB8

OMIM

177046 (Human)

UniProt

AAH01114 (Human)

Additional LMP7/PSMB8 Products

Product Documents for LMP7/PSMB8 Antibody (1B3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LMP7/PSMB8 Antibody (1B3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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