LMX1A Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41193
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Predicted:
Chicken (92%). Backed by our 100% Guarantee.
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LMX1A. The immunogen is located within amino acids 300 - 350 of LMX1A. Amino Acid Squence: CPQMPGDHMHPYGAEPL
Specificity
At least two isoforms are known to exist. This antibody is predicted to not cross-react with LMX1B.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for LMX1A Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: LMX1A Antibody - BSA Free [NBP2-41193] -
Immunocytochemistry/ Immunofluorescence: LMX1A Antibody - BSA Free [NBP2-41193] - Validation of LMX1A in HeLa Cells.Immunofluorescent analysis of methanol-fixed HeLa cells labeling LMX1A at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody followed by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] -
Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Validation in Human Skeletal Muscle. Loading: 10 ul of lysates per lane.Antibodies: LMX1A, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] -
Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Validation in Mouse Skeletal Muscle.Loading: 10 ul of lysates per lane.Antibodies: LMX1A, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Applications for LMX1A Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
2.5 ug/mL
Immunohistochemistry-Paraffin
2.5 ug/ml
Western Blot
1 - 2 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LMX1A
Alternate Names
LIM homeobox transcription factor 1, alpha, LMX1.1
Gene Symbol
LMX1A
Additional LMX1A Products
Product Documents for LMX1A Antibody - BSA Free
Product Specific Notices for LMX1A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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