Loricrin Antibody (JE40-03)

Immunohistochemistry: Loricrin Antibody (JE40-03) [NBP3-32544]

Immunohistochemistry: Loricrin Antibody (JE40-03) [NBP3-32544] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Loricrin antibody (NBP3-32544) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32544) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry: Loricrin Antibody (JE40-03) [NBP3-32544]

Immunohistochemistry: Loricrin Antibody (JE40-03) [NBP3-32544] - Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Loricrin with Rabbit anti-Loricrin antibody (NBP3-32544) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32544, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).