LOX Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2527

Novus Biologicals, part of Bio-Techne
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NB100-2527
NB100-2527SS
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Cited:

Human, Mouse, Rat, Porcine, Feline

Predicted:

Bovine (92%), Chicken (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Lysyl Oxidase/LOX Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region of the human LOX protein (within residues 200-300). [Swiss-Prot P28300].

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27169768). Immunogen displays the following percentage of sequence identity for non-tested species: bovine (92%), chicken (92%), xenopus (85%). Porcine reactivity reported in scientific literature (PMID: 30036726).

Localization

Secreted, extracellular space.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LOX Antibody

Western Blot: LOX Antibody [NB100-2527]

Western Blot: LOX Antibody [NB100-2527]

Western Blot: LOX Antibody [NB100-2527] - LOX secretion and activity in response to tumor cell irradiation. Western blot of LOX zymogen (50 kDa) and active form (32 kDa) in conditioned media derived from irradiated A549 lung carcinoma cells 16-20 h after irradiation and quantification of band intensities from 3 independent experiments. Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-532) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527]

Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527]

Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527] - Expression of Lox and Loxl1-Loxl4 mRNAs in orthotopic rat prostate tumours and in the surrounding normal rat prostate tissue. Representative sections from a 10-day orthotopic AT-1 rat prostate tumour (T), tumour-adjacent normal rat prostate tissue (N), and tumour-free control rat prostate tissue stained for LOX (inserts show LOX staining in higher magnifications). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep19608), licensed under a CC-BY license.
Simple Western: LOX Antibody [NB100-2527]

Simple Western: LOX Antibody [NB100-2527]

Simple Western: LOX Antibody [NB100-2527] - Simple Western shows a specific band for LOX in 1.0 mg/ml of HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
View 8 more images

Applications for LOX Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100-1:250

Immunohistochemistry-Paraffin

1:100-1:250

Simple Western

1:25

Western Blot

0.5-4ug/ml
Application Notes
This LOX antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, and Western blot. In Western blot, bands are observed ~58 kDa representing glycosylated Lox and ~32 kDa representing the mature, secreted form of Lox. In ICC/IF nuclear staining was observed in HeLa cells, which is expected for the mature form of LOX according to published literature (PMID 17287363 and 10996848).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 5 reviews rated 4 using NB100-2527 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS pH 7.2

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lysyl Oxidase/LOX

LOX (lysyl oxidase) is a copper-dependent amine oxidase enzyme responsible for post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOX is synthesized as an inactive 50 kD proenzyme that is N-glycosylated in ER/Golgi complex and then secreted into the extracellular environment, where it is processed by pro-collagen C-proteinases (mammalian Tolloids and BMP-1) into an active enzyme (28-32 kD) and an 18 kD propeptide (LOX-PP) which can be N-glycosylated intracellularly into a ~35 kD form. LOX activity is critical in maintaining the tensile as well as elastic features of the connective tissues of skeletal, pulmonary and cardiovascular systems. LOX deficiency leads to intra-gestational lethality in LOX-/- mice wherein the pups exhibits aortic aneurysms/tortuosity/rupture. LOX expression is highly regulated during normal development. Aberrant expression or activity has been documented in a range of diseases predominantly associated with the ECM such as Cutis laxa or OHS (occipital horn syndrome), Menkes' syndrome, myocardial ischaemia/heart failure, atherosclerosis, scleroderma, liver cirrhosis, Pseudoexfoliation syndrome and glaucoma, Alzheimer's/non-Alzheimer's dementia, Wilson's disease, primary biliary cirrhosis as well as in many type of cancers, wherein LOX implicates in promoting tumor cell invasion and metastasis.

Long Name

Lysyl Oxidase

Alternate Names

AAT10

Gene Symbol

LOX

Additional Lysyl Oxidase/LOX Products

Product Documents for LOX Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LOX Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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