LOX Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2530
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine
Cited:
Human, Mouse
Predicted:
Chicken (92%), Xenopus (92%), Zebrafish (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A cocktail of two synthetic peptides; one made to a region of the human LOX protein within residues 300-350 (NB100-2528) and one within residues 200-300 (NB100-2527). [Swiss-Prot P28300]
Reactivity Notes
92% sequence identity with chicken, Zebrafish, and Xenopus proteins.
Localization
Cytosolic and nuclear.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LOX Antibody - BSA Free
Western Blot: LOX AntibodyBSA Free [NB100-2530]
Western Blot: LOX Antibody [NB100-2530] - Detection of LOX in mouse kidney lysate using NB 100-2530 (1:1,000). ECL detection was 15 sec.Immunocytochemistry/ Immunofluorescence: LOX Antibody - BSA Free [NB100-2530]
Immunocytochemistry/Immunofluorescence: LOX Antibody [NB100-2530] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-LOX [NB100-2530] at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.Immunohistochemistry-Paraffin: LOX Antibody - BSA Free [NB100-2530]
Immunohistochemistry-Paraffin: LOX Antibody [NB100-2530] - Lysyl Oxidase (LOX) expression in malignant and non-malignant human prostate tissue. Consecutive sections from non-malignant prostate tissue stained with in situ hybridization or immunohistochemistry for LOX. Note that mRNA (brown dots) and protein (brown) expression in individual glands were related, in the stroma in contrast mRNA was generally low but protein often detected (original magnifications x200). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0140985), licensed under a CC-BY license.Applications for LOX Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Simple Western
1:100
Western Blot
1:500-1:1000
Application Notes
This LOX antibody is useful for Western blot and Immunohistochemistry paraffin embedded sections. In Western blot a band is seen at ~58 kDa. This is a highly glycosylated protein; therefore one may see bands at ~32 kDa, ~50 kDa and ~58 kDa representing the mature (secreted) form, the pro form and the glycosylated forms respectively.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Lysyl Oxidase/LOX
Long Name
Lysyl Oxidase
Alternate Names
AAT10
Gene Symbol
LOX
UniProt
Additional Lysyl Oxidase/LOX Products
Product Documents for LOX Antibody - BSA Free
Product Specific Notices for LOX Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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