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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine

Cited:

Human, Mouse

Predicted:

Chicken (92%), Xenopus (92%), Zebrafish (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

A cocktail of two synthetic peptides; one made to a region of the human LOX protein within residues 300-350 (NB100-2528) and one within residues 200-300 (NB100-2527). [Swiss-Prot P28300]

Reactivity Notes

92% sequence identity with chicken, Zebrafish, and Xenopus proteins.

Localization

Cytosolic and nuclear.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LOX Antibody - BSA Free

Western Blot: LOX AntibodyBSA Free [NB100-2530]

Western Blot: LOX AntibodyBSA Free [NB100-2530]

Western Blot: LOX Antibody [NB100-2530] - Detection of LOX in mouse kidney lysate using NB 100-2530 (1:1,000). ECL detection was 15 sec.
Immunocytochemistry/ Immunofluorescence: LOX Antibody - BSA Free [NB100-2530]

Immunocytochemistry/ Immunofluorescence: LOX Antibody - BSA Free [NB100-2530]

Immunocytochemistry/Immunofluorescence: LOX Antibody [NB100-2530] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-LOX [NB100-2530] at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: LOX Antibody - BSA Free [NB100-2530]

Immunohistochemistry-Paraffin: LOX Antibody - BSA Free [NB100-2530]

Immunohistochemistry-Paraffin: LOX Antibody [NB100-2530] - Lysyl Oxidase (LOX) expression in malignant and non-malignant human prostate tissue. Consecutive sections from non-malignant prostate tissue stained with in situ hybridization or immunohistochemistry for LOX. Note that mRNA (brown dots) and protein (brown) expression in individual glands were related, in the stroma in contrast mRNA was generally low but protein often detected (original magnifications x200). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0140985), licensed under a CC-BY license.

Applications for LOX Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Simple Western

1:100

Western Blot

1:500-1:1000
Application Notes
This LOX antibody is useful for Western blot and Immunohistochemistry paraffin embedded sections. In Western blot a band is seen at ~58 kDa. This is a highly glycosylated protein; therefore one may see bands at ~32 kDa, ~50 kDa and ~58 kDa representing the mature (secreted) form, the pro form and the glycosylated forms respectively.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lysyl Oxidase/LOX

LOX (lysyl oxidase) is a copper-dependent amine oxidase enzyme responsible for post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOX is synthesized as an inactive 50 kD proenzyme that is N-glycosylated in ER/Golgi complex and then secreted into extracellular environment, where it is processed by pro-collagen C-proteinases (mammalian Tolloids and BMP-1) into an active enzyme (28-32 kD) and an 18 kD propeptide (LOX-PP) which can be N-glycosylated intracellularly into a ~35 kD form. LOX activity is critical in maintaining the tensile as well as elastic features of the connective tissues of skeletal, pulmonary and cardiovascular systems. LOX deficiency leads to intra-gestational lethality in LOX-/- mice wherein the pups exhibits aortic aneurysms/tortuosity/rupture. LOX expression is highly regulated during normal development; and, its aberrant expression or activity has been documented in a range of diseases predominantly associated with the ECM such as Cutis laxa or OHS (occipital horn syndrome), Menkes' syndrome, myocardial ischaemia/heart failure, atherosclerosis, scleroderma, liver cirrhosis, Pseudoexfoliation syndrome and glaucoma, Alzheimer's/non-Alzheimer's dementia, Wilson's disease, primary biliary cirrhosis as well as in many type of cancers, wherein LOX implicates in promoting tumor cell invasion and metastasis.

Long Name

Lysyl Oxidase

Alternate Names

AAT10

Entrez Gene IDs

4015 (Human); 16948 (Mouse); 24914 (Rat)

Gene Symbol

LOX

UniProt

Additional Lysyl Oxidase/LOX Products

Product Documents for LOX Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LOX Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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