LOX propeptide Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30327

Novus Biologicals, part of Bio-Techne
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NBP1-30327
NBP1-30327SS
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Simple Western, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all LOX propeptide Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human LOX propeptide (within residues 118-168). [Swiss-Prot# P28300]

Specificity

This antibody recognizes the glycosylated propeptide form (~35 kDa) and the proenzyme form (~50 kDa).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LOX propeptide Antibody - BSA Free

Western Blot: LOX propeptide AntibodyBSA Free [NBP1-30327]

Western Blot: LOX propeptide AntibodyBSA Free [NBP1-30327]

Western Blot: LOX propeptide Antibody [NBP1-30327] - LOX-PP reduces CIN85 mono-ubiquitination and ability to interact with c-Cbl. HEK293T cells were transfected with AMAP1-FLAG, CIN85, HA-c-Cbl, HA-ubiquitin and LOX-PP-GST (PP-GST) as indicated and subjected to a ubiquitination assay. FLAG-tagged AMAP1 was immunoprecipitated and total whole cell extracts were subjected to WB with the indicated antibodies (lower panel). Data were quantified and relative mono-ubiquitination of AMAP1 with and without LOX-PP was determined by averaging the results of three independent experiments. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0077288), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: LOX propeptide Antibody - BSA Free [NBP1-30327]

Immunocytochemistry/ Immunofluorescence: LOX propeptide Antibody - BSA Free [NBP1-30327]

Immunocytochemistry/Immunofluorescence: LOX propeptide Antibody [NBP1-30327] - Staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).
Flow Cytometry: LOX propeptide Antibody - BSA Free [NBP1-30327]

Flow Cytometry: LOX propeptide Antibody - BSA Free [NBP1-30327]

Flow Cytometry: LOX propeptide Antibody [NBP1-30327] - An intracellular stain was performed on HeLa with LOX propeptide Antibody NBP1-30327 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, .
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Applications for LOX propeptide Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1:200

Immunocytochemistry/ Immunofluorescence

1:200

Immunoprecipitation

1:10-1:500. Use reported in scientific literature (PMID 21536655)

Simple Western

1:200

Western Blot

0.5 ug/ml
Application Notes
In WB a band can be seen at ~35 kDa (glycosylated propeptide form) and at ~50 kDa (proenzyme form).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LOX propeptide

Lysyl oxidase (LOX) is a critical enzyme in extracellular matrix biosynthesis as well as in the maintainance of normal bone phenotype. It is synthesized/secreted as a 50-kD proenzyme 'pro-LOX' which is then processed to 30-kD mature enzyme 'LOX' and 18-kD lysyl oxidase propeptide 'LOX-PP' by extracellular procollagen C-proteinases (BMP1, Tll1, and Tll2). LOX-PP has been proposed to implicate in the maintenance of inactive state of LOX within the secretory pathway and to act as intramolecular chaperones which facilitate the accurate protein folding/targeting to correct destinations. LOX-PP involves tumor suppression by inhibiting RAS-dependent cellular transformation and its expression has also been associated with inhibition of anchorage-independent progression as well as migration and growth suppression xenografts in Her-2/neu-driven breast cancer. LOX-PP also inhibits PI3K/AKT and ERK1/2 MAP kinase pathways, as well as the levels of downstream NF-kB/cyclin D1 signals in certain cancers - breast, pancreatic, lung, prostate and oral cancers. LOX-PP has also been shown to inhibit DNA synthesis in rat's VSMCs cultures, osteoblast proliferation and FGF-2 signaling.

Alternate Names

lysyl oxidaseEC 1.4.3.13, MGC105112, protein-lysine 6-oxidase

Entrez Gene IDs

4015 (Human)

Gene Symbol

LOX

UniProt

P28300 (Human)

Additional LOX propeptide Products

Product Documents for LOX propeptide Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LOX propeptide Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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