LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72519

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1G6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all LPAR1/LPA1/EDG-2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LPAR1(NP_001392) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

40.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free

Western Blot: LPAR1/LPA1/EDG-2 Antibody (OTI1G6)Azide and BSA Free [NBP2-72519]

Western Blot: LPAR1/LPA1/EDG-2 Antibody (OTI1G6)Azide and BSA Free [NBP2-72519]

Western Blot: LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free [NBP2-72519] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EDG2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EDG2.
Flow Cytometry: LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free [NBP2-72519]

Flow Cytometry: LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free [NBP2-72519]

Flow Cytometry: LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free [NBP2-72519] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EDG2.
Flow Cytometry: LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free [NBP2-72519]

Flow Cytometry: LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free [NBP2-72519]

Flow Cytometry: LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free [NBP2-72519] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-EDG2 antibody, and then analyzed by flow cytometry.

Applications for LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LPAR1/LPA1/EDG-2

EDG2 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. There are eight known members of the EDG receptor family and they are implicated in mediating growth-related effects such as induction of cellular proliferation, alterations in differentiation and survival, and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C, and several proteins not used within 12 hours. Edg2 has been reported in most human tissues, and is especially abundant in brain cortical regions. ESTs have been isolated from bone, brain, breast, connective tissue, embryo, heart/melanocyte/uterus, lung, prostate and uterus libraries.

Long Name

Lysophosphatidic Acid Receptor 1

Alternate Names

EDG2, GPCR26, GPR26, LPA1, rec.1.3, vzg-1

Gene Symbol

LPAR1

Additional LPAR1/LPA1/EDG-2 Products

Product Documents for LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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