LPAR1/LPA1/EDG-2 Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-03363CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-03363AF350

Alexa Fluor 405

NBP1-03363AF405

Alexa Fluor 488

NBP1-03363AF488

Alexa Fluor 532

NBP1-03363AF532

Alexa Fluor 594

NBP1-03363AF594

Alexa Fluor 647

NBP1-03363AF647

Alexa Fluor 700

NBP1-03363AF700

Alexa Fluor 750

NBP1-03363AF750

Biotin

NBP1-03363B

DyLight 350

NBP1-03363UV

DyLight 405

NBP1-03363V

DyLight 488

NBP1-03363G

DyLight 550

NBP1-03363R

DyLight 594

NBP1-03363DL594

DyLight 650

NBP1-03363C

DyLight 680

NBP1-03363FR

DyLight 755

NBP1-03363IR

FITC

NBP1-03363F

HRP

NBP1-03363H

Janelia Fluor 525

NBP1-03363JF525

Janelia Fluor 549

NBP1-03363JF549

Janelia Fluor 646

NBP1-03363JF646

Janelia Fluor 669

NBP1-03363JF669

mFluor Violet 450 SE

NBP1-03363MFV450

mFluor Violet 500 SE

NBP1-03363MFV500

mFluor Violet 610 SE

NBP1-03363MFV610

Forumulation

Catalog #

BSA Free

NBP1-03363

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Predicted:

Bovine (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all LPAR1/LPA1/EDG-2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human EDG2 (within residues 200-300). [Swiss-Prot# Q92633]

Reactivity Notes

Predicted to react with sheep, and bovine based on 100% sequence homology. Positive staining in IHC-Fr/IF with 16 um cryostat sections of embryonic day 6 (E6) chicken brain was reported by a customer. Mouse reactivity reported in scientific literature (PMID: 24147068).

Localization

Cell membrane; Multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for LPAR1/LPA1/EDG-2 Antibody [CoraFluor™ 1]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: LPAR1/LPA1/EDG-2

EDG2 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. There are eight known members of the EDG receptor family and they are implicated in mediating growth-related effects such as induction of cellular proliferation, alterations in differentiation and survival, and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C, and several proteins not used within 12 hours. Edg2 has been reported in most human tissues, and is especially abundant in brain cortical regions. ESTs have been isolated from bone, brain, breast, connective tissue, embryo, heart/melanocyte/uterus, lung, prostate and uterus libraries.

Long Name

Lysophosphatidic Acid Receptor 1

Alternate Names

EDG2, GPCR26, GPR26, LPA1, rec.1.3, vzg-1

Gene Symbol

LPAR1

Additional LPAR1/LPA1/EDG-2 Products

Product Documents for LPAR1/LPA1/EDG-2 Antibody [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for LPAR1/LPA1/EDG-2 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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