LPAR2/LPA2/EDG-4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84904

Novus Biologicals, part of Bio-Techne
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NBP1-84904
NBP1-84904-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LPAR2/LPA2/EDG-4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLLDGLGCESCNVLAVEKYFLLLAEANSLVNAAVYSCRDAEMRRTFRRLLCCACLRQSTRESVHYTSSAQGGASTRIMLPENGHPLMDSTL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (87%). Human reactivity reported in scientific literature (PMID: 25002680).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LPAR2/LPA2/EDG-4 Antibody

Western Blot: LPAR2/LPA2/EDG-4 Antibody [NBP1-84904]

Western Blot: LPAR2/LPA2/EDG-4 Antibody [NBP1-84904]

Western Blot: LPAR2/LPA2/EDG-4 Antibody [NBP1-84904] - Representative western blot and densitometric analysis of Caco-2, HT-29 and HCT-116 cells using specific antibodies against LPAR2/LPA2/EDG-4. Caco-2 cells express high levels of LPAR2/LPA2/EDG-4. The average scores +/- SEM is shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26418031/) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: LPAR2/LPA2/EDG-4 Antibody [NBP1-84904]

Immunohistochemistry-Paraffin: LPAR2/LPA2/EDG-4 Antibody [NBP1-84904]

Immunohistochemistry-Paraffin: LPAR2/LPA2/EDG-4 Antibody [NBP1-84904] - Staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.

Applications for LPAR2/LPA2/EDG-4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 26418031). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LPAR2/LPA2/EDG-4

Edg4 is a Lysophospholipid/Lysosphingolipid Receptor activated by phosphatidic acid (PA) and lysophosphatidic acid (LPA). It enhances the transcription of serum response element (SRE) reporter genes. In CD4(+) T cells, Edg4 may be involved in the control of interleukin-2 secretion. Edg4 expression has been documented in leukocytes, pancreas, spleen, thymus, prostate, and testis. Edg4 expression has also been seen in many cancer cell lines, including those of brain, blood, breast, ovary, cervix, colon, skin, and thyroid. ESTs have been isolated from B-cell/lung/testis, blood, brain, embryo, heart/melanocyte/uterus, liver, placenta, prostate, and skin libraries.

Long Name

Lysophosphatidic Acid Receptor 2

Alternate Names

EDG4, LPA2, LPAR2

Gene Symbol

LPAR2

Additional LPAR2/LPA2/EDG-4 Products

Product Documents for LPAR2/LPA2/EDG-4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LPAR2/LPA2/EDG-4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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