LRH-1/NR5A2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42513

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Porcine

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all LRH-1/NR5A2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TDYDRSPFVTSPIS corresponding to internal region according to NP_003813.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_995582.1; NP_003813.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LRH-1/NR5A2 Antibody

Western Blot: LRH-1/NR5A2 Antibody [NBP2-42513]

Western Blot: LRH-1/NR5A2 Antibody [NBP2-42513]

Western Blot: LRH-1/NR5A2 Antibody [NBP2-42513] - Staining of Pig Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: LRH-1/NR5A2 Antibody [NBP2-42513]

Western Blot: LRH-1/NR5A2 Antibody [NBP2-42513]

Western Blot: LRH-1/NR5A2 Antibody [NBP2-42513] - Analysis of HepG2 nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for LRH-1/NR5A2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3-1 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in nuclear lysates of HeLa and HepG2 (calculated MW of 56.5 kDa band according to NP_003813.1).Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Liver and NIH-3T3 cell line for this particular batch.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LRH-1/NR5A2

Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells.

Long Name

Liver Receptor Homolog 1/Nuclear Receptor Subfamily 5, Group A, Member 2

Alternate Names

B1F2, FTZ-F1 beta, LRH1, NR5A2

Entrez Gene IDs

2494 (Human); 26424 (Mouse); 60349 (Rat)

Gene Symbol

NR5A2

Additional LRH-1/NR5A2 Products

Product Documents for LRH-1/NR5A2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LRH-1/NR5A2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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