LRP-6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1475

Novus Biologicals, part of Bio-Techne
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NB100-1475
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all LRP-6 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CMTSVATAKGYTSDL corresponding to internal region according to NP_002327.2.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 20303298).

Specificity

This antibody is expected to recognise an epitope corresponding to aa 1546-1560 of human LRP6 protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LRP-6 Antibody

Immunohistochemistry-Paraffin: LRP-6 Antibody [NB100-1475]

Immunohistochemistry-Paraffin: LRP-6 Antibody [NB100-1475]

Immunohistochemistry-Paraffin: LRP-6 Antibody [NB100-1475] - Analysis of paraffin embedded Human Kidney using this antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry: LRP-6 Antibody [NB100-1475]

Flow Cytometry: LRP-6 Antibody [NB100-1475]

Flow Cytometry: LRP-6 Antibody [NB100-1475] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for LRP-6 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunohistochemistry

4 - 6 ug/mL

Immunohistochemistry-Paraffin

4 - 6 ug/mL

Peptide ELISA

Detection limit 1:64000
Application Notes
Use in Western blot reported in scientific literature (PMID: 20303298).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LRP-6

LRP6 is essential for the Wnt/beta catenin signaling pathway, probably by acting as a coreceptor together with Frizzled for Wnt. It is a specific, high-affinity receptor for DKK1 and DKK2, but not DKK3. The interaction with DKK1 blocks LRP6-mediated Wnt/beta catenin signaling via LRP6 removal via Kremen proteins-mediated endocytosis.

Long Name

LDL Receptor-related Protein 6

Alternate Names

ADCAD2, FLJ90062, FLJ90421, LRP6

Entrez Gene IDs

4040 (Human); 16974 (Mouse)

Gene Symbol

LRP6

UniProt

O75581

Additional LRP-6 Products

Product Documents for LRP-6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LRP-6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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