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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 545818

Concentration

1.0 mg/ml

Product Summary for LRP2 Antibody (545818) [Unconjugated]

Immunogen

Mouse myeloma cell line NS0-derived partial recombinant human Megalin/LRP2 protein (amino acids 3510-3964)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for LRP2 Antibody (545818) [Unconjugated]

Immunohistochemistry-Paraffin: LRP2 Antibody (545818) [Unconjugated] [MAB95781] - Megalin/LRP2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Megalin/LRP2 Monoclonal Antibody (Catalog # MAB95781) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membrane of epithelial cells in convoluted tubules.

Applications for LRP2 Antibody (545818) [Unconjugated]

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Megalin/LRP2

Megalin/gp330, an approx. 600 kDa transmembrane glycoprotein is a large cell surface receptor that mediates the binding and internalization of a number of structurally and functionally distinct ligands from the lipoprotein and protease inhibitor families.

Long Name

LDL Receptor-related Protein 2

Alternate Names

DBS, gp330, LRP-2, LRP2

Gene Symbol

LRP2

Additional Megalin/LRP2 Products

Product Documents for LRP2 Antibody (545818) [Unconjugated]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LRP2 Antibody (545818) [Unconjugated]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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