LRPAP Antibody (009)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90513
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513]](https://resources.bio-techne.com/images/products/LRPAP-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90513-img0004.jpg "Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513]")
![Immunoprecipitation: LRPAP Antibody (009) [NBP2-90513]](https://resources.bio-techne.com/images/products/LRPAP-Antibody-009-Immunoprecipitation-NBP2-90513-img0006.jpg "Immunoprecipitation: LRPAP Antibody (009) [NBP2-90513]")
![Western Blot: LRPAP Antibody (009) [NBP2-90513]](https://resources.bio-techne.com/images/products/LRPAP-Antibody-009-Western-Blot-NBP2-90513-img0003.jpg "Western Blot: LRPAP Antibody (009) [NBP2-90513]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 009
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for LRPAP Antibody (009)
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LRPAP (Accession#: NP_038615.2; Gln 29-Leu 360).
Specificity
No cross-reactivity in ELISA with: Human LRPAP1
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for LRPAP Antibody (009)
Western Blot: LRPAP Antibody (009) [NBP2-90513]
Western Blot: LRPAP Antibody (009) [NBP2-90513] - Anti-LRPAP rabbit monoclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa![Immunocytochemistry/ Immunofluorescence: LRPAP Antibody (009) [NBP2-90513]](https://resources.bio-techne.com/images/products/LRPAP-Antibody-009-Immunocytochemistry-Immunofluorescence-NBP2-90513-img0002.jpg "Immunocytochemistry/ Immunofluorescence: LRPAP Antibody (009) [NBP2-90513]")
Immunocytochemistry/ Immunofluorescence: LRPAP Antibody (009) [NBP2-90513]
Immunocytochemistry/Immunofluorescence: LRPAP Antibody (009) [NBP2-90513] - Immunofluorescence staining of mouse LRPAP in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-mouse LRPAP monoclonal antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Endoplasmic reticulum.![Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513]](https://resources.bio-techne.com/images/products/LRPAP-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90513-img0005.jpg "Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513]")
Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513]
Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513] - Immunochemical staining of mouse LRPAP in mouse brain with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections. The image showing staining of nerve cells.Applications for LRPAP Antibody (009)
Application
Recommended Usage
ELISA
1:25000-1:50000
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
0.2-1 uL/mg of lysate
Western Blot
1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LRPAP
Long Name
Low Density Lipoprotein-Related Protein-associated Protein
Alternate Names
A2MRAP, A2RAP, HBP44, LRPAP1, RAP
Gene Symbol
LRPAP1
Additional LRPAP Products
Product Documents for LRPAP Antibody (009)
Product Specific Notices for LRPAP Antibody (009)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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