LRPAP Antibody (4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89796
Recombinant Monoclonal Antibody
![Western Blot: LRPAP Antibody (4) [NBP2-89796]](https://resources.bio-techne.com/images/products/LRPAP-Antibody-4-Western-Blot-NBP2-89796-img0002.jpg "Western Blot: LRPAP Antibody (4) [NBP2-89796]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for LRPAP Antibody (4)
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human LRPAP (Tyr 35-Leu 357; Accession#: NP_002328.1).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for LRPAP Antibody (4)
Western Blot: LRPAP Antibody (4) [NBP2-89796]
Western Blot: LRPAP Antibody (4) [NBP2-89796] - Lane A: U87-MG Whole Cell Lysate Lane B: PC-3 Whole Cell LysateLane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:41 kDa Observed band size:41 kDa![Immunocytochemistry/ Immunofluorescence: LRPAP Antibody (4) [NBP2-89796]](https://resources.bio-techne.com/images/products/LRPAP-Antibody-4-Immunocytochemistry-Immunofluorescence-NBP2-89796-img0001.jpg "Immunocytochemistry/ Immunofluorescence: LRPAP Antibody (4) [NBP2-89796]")
Immunocytochemistry/ Immunofluorescence: LRPAP Antibody (4) [NBP2-89796]
Immunocytochemistry/Immunofluorescence: LRPAP Antibody (4) [NBP2-89796] - Staining of Human LRPAP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LRPAP1 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.![Immunoprecipitation: LRPAP Antibody (4) [NBP2-89796]](https://resources.bio-techne.com/images/products/LRPAP-Antibody-4-Immunoprecipitation-NBP2-89796-img0003.jpg "Immunoprecipitation: LRPAP Antibody (4) [NBP2-89796]")
Immunoprecipitation: LRPAP Antibody (4) [NBP2-89796]
Immunoprecipitation: LRPAP Antibody (4) [NBP2-89796] - Lane A:0.5 mg 293T Whole Cell Lysate 2 uL anti-LRPAP1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-LRPAP1 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 41 kDa Observed band size: 41 kDaApplications for LRPAP Antibody (4)
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LRPAP
Long Name
Low Density Lipoprotein-Related Protein-associated Protein
Alternate Names
A2MRAP, A2RAP, HBP44, LRPAP1, RAP
Gene Symbol
LRPAP1
Additional LRPAP Products
Product Documents for LRPAP Antibody (4)
Product Specific Notices for LRPAP Antibody (4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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