LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12047

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Product Datasheet / COA

Recombinant Monoclonal Antibody

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NBP3-12047AF350

Alexa Fluor 405

NBP3-12047AF405

Alexa Fluor 488

NBP3-12047AF488

Alexa Fluor 532

NBP3-12047AF532

Alexa Fluor 594

NBP3-12047AF594

Alexa Fluor 647

NBP3-12047AF647

Alexa Fluor 700

NBP3-12047AF700

Alexa Fluor 750

NBP3-12047AF750

Biotin

NBP3-12047B

CoraFluor 1

NBP3-12047CL1

DyLight 350

NBP3-12047UV

DyLight 405

NBP3-12047V

DyLight 488

NBP3-12047G

DyLight 550

NBP3-12047R

DyLight 594

NBP3-12047DL594

DyLight 650

NBP3-12047C

DyLight 680

NBP3-12047FR

DyLight 755

NBP3-12047IR

FITC

NBP3-12047F

HRP

NBP3-12047H

Janelia Fluor 525

NBP3-12047JF525

Janelia Fluor 549

NBP3-12047JF549

Janelia Fluor 585

NBP3-12047JF585

Janelia Fluor 635

NBP3-12047JF635

Janelia Fluor 646

NBP3-12047JF646

Janelia Fluor 669

NBP3-12047JF669

mFluor Violet 450 SE

NBP3-12047MFV450

mFluor Violet 500 SE

NBP3-12047MFV500

mFluor Violet 610 SE

NBP3-12047MFV610

PerCP

NBP3-12047PCP

Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 7F1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all LRPAP Antibodies »

Product Summary for LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free

Immunogen

This antibody was raised by immunising mice with human placental LRPAP.

Specificity

This antibody is specific for human LRPAP. It does not cross-react with rabbit or murine LRPAP.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free

Western Blot: LRPAP Antibody (7F1)ChimericAzide and BSA Free [NBP3-12047]

Western Blot: LRPAP Antibody (7F1) - Chimeric [NBP3-12047] - Staining in HeLa(A) (0.1ug/ml), A549 (B) (0.01ug/ml), HepG2 (C) (0.01ug/ml), HEK293 (D) (0.03ug/ml), MCF7 (E) (0.03ug/ml), Caco-2 (F) (0.001ug/ml) and U251 (G) (0.001ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAP (NBP3-12047) at 0.5 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunocytochemistry/ Immunofluorescence: LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free [NBP3-12047]

Immunocytochemistry/Immunofluorescence: LRPAP Antibody (7F1) - Chimeric [NBP3-12047] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 7F1 (NBP3-12047) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12047, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: LRPAP

Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330

Long Name

Low Density Lipoprotein-Related Protein-associated Protein

Alternate Names

A2MRAP, A2RAP, HBP44, LRPAP1, RAP

Gene Symbol

LRPAP1

Additional LRPAP Products

Product Documents for LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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