LRRC28 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48706
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: NIHLKGLPSYLYNKVIGCSGCGAPIQVSEVKLLSFSSGQRTVFLPAEVKAIGTEHDHVLPLQELAMRGLYHTYHSLLKDLNFL
Reactivity Notes
Mouse (83%), Rat (81%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LRRC28 Antibody
Western Blot: LRRC28 Antibody [NBP2-48706]
Western Blot: LRRC28 Antibody [NBP2-48706] - Analysis in control (vector only transfected HEK293T lysate) and LRRC28 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: LRRC28 Antibody [NBP2-48706]
Immunocytochemistry/Immunofluorescence: LRRC28 Antibody [NBP2-48706] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus & the Golgi apparatus.Immunohistochemistry-Paraffin: LRRC28 Antibody [NBP2-48706]
Immunohistochemistry-Paraffin: LRRC28 Antibody [NBP2-48706] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.Applications for LRRC28 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LRRC28
Alternate Names
FLJ34269, FLJ45242, leucine rich repeat containing 28, leucine-rich repeat-containing protein 28, MGC24976
Gene Symbol
LRRC28
Additional LRRC28 Products
Product Documents for LRRC28 Antibody
Product Specific Notices for LRRC28 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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