LRRC32/GARP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24664

Novus Biologicals, part of Bio-Techne
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NBP2-24664
NBP2-24664SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Western Blot

Cited:

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all LRRC32/GARP Antibodies »

Product Specifications

Immunogen

A portion of amino acids 250-300 of human GARP/LRRC32 was used as the immunogen.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31198639).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LRRC32/GARP Antibody

Western Blot: LRRC32/GARP Antibody [NBP2-24664]

Western Blot: LRRC32/GARP Antibody [NBP2-24664]

Western Blot: LRRC32/GARP Antibody [NBP2-24664] - Analysis of GARP protein expression; (t test, * P < 0.05). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31198639/) licensed under a CC-BY license.
Flow Cytometry: LRRC32/GARP Antibody [NBP2-24664]

Flow Cytometry: LRRC32/GARP Antibody [NBP2-24664]

Flow Cytometry: LRRC32/GARP Antibody [NBP2-24664] - BALB/c splenocytes stained with anti-GARP (NBP2-24664), with a FITC conjugated secondary (20302), and anti-mouse CD4 PE (IMG-5922D). Viable cells in the lymphocyte gate were used for analysis. Novus's cell surface flow kit (10084K) was used for this test .
Western Blot: LRRC32/GARP Antibody [NBP2-24664]

Western Blot: LRRC32/GARP Antibody [NBP2-24664]

Western Blot: LRRC32/GARP Antibody [NBP2-24664] - Analysis of GARP/LRRC32 in mouse thymus lysate in the (1) absence and (2) presence of immunizing peptide using this antibody. 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for LRRC32/GARP Antibody

Application
Recommended Usage

Flow Cytometry

1-3 ug/ml

Western Blot

0.5-1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.1 ml PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LRRC32/GARP

GARP, also known as leucine rich repeat containing 32, is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region. GARP was identified based on the analysis of gene expression profiling of Treg and Th cells following TCR stimulation. Since GARP is expressed in CD4+, CD25+, FoxP3+ cells (Tregs), it may be a candidate for cell surface marker for Treg cells.

Long Name

Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant

Alternate Names

D11S833E, GARP, Garpin

Gene Symbol

LRRC32

UniProt

Q14392

Additional LRRC32/GARP Products

Product Documents for LRRC32/GARP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LRRC32/GARP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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