LRRFIP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71835

Novus Biologicals, part of Bio-Techne
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NBP1-71835
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all LRRFIP1 Antibodies »

Product Summary for LRRFIP1 Antibody

Immunogen

The immunogen for this product maps to a region between residue 500 and 550 of human Leucine Rich Repeat (in FLII) Interacting Protein 1 using the numbering given in entry NP_001131024.1 (GeneID 9208).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LRRFIP1 Antibody

Western Blot: LRRFIP1 Antibody [NBP1-71835]

Western Blot: LRRFIP1 Antibody [NBP1-71835]

Western Blot: LRRFIP1 Antibody [NBP1-71835] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (50 ug) cells. Affinity purified rabbit anti-LRRFIP1 antibody used for WB at 0.04 ug/mL (A) and 0.4 ug/mL (B) and used for IP at 6 ug/mg lysate. LRRFIP1 was also immunoprecipitated by rabbit anti-LRRFIP1 antibodies NBP1-71834 and NBP1-71836, which recognize other epitopes.
Immunocytochemistry/ Immunofluorescence: LRRFIP1 Antibody [NBP1-71835]

Immunocytochemistry/ Immunofluorescence: LRRFIP1 Antibody [NBP1-71835]

Immunocytochemistry/Immunofluorescence: LRRFIP1 Antibody [NBP1-71835] - LRRFIP1 in human fibroblasts (Green). 1:250 dilution in 1X PBS/1% BSA/0.3% Triton X-100. 16 hours, 4C. Image submitted by a verified customer review.
Immunoprecipitation: LRRFIP1 Antibody [NBP1-71835]

Immunoprecipitation: LRRFIP1 Antibody [NBP1-71835]

Immunoprecipitation: LRRFIP1 Antibody [NBP1-71835] - LRRFIP1 IP in B-cell whole cell lysate. 2 ug antibody : 500 ug whole cell lysate. Same antibody used at 1:2000 for Western blot. Image submitted by a verified customer review.

Applications for LRRFIP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

From verified customer review.

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.3 using NBP1-71835 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LRRFIP1

Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA

Alternate Names

FLAP-1, FLIIAP1, GC-binding factor 2, GCF2, GCF-2, HUFI-1, leucine rich repeat (in FLII) interacting protein 1, leucine-rich repeat flightless-interacting protein 1, LRR FLII-interacting protein 1, MGC10947, MGC119738, MGC119739, NEDD8-conjugating enzyme, TRIPTAR RNA-interacting protein

Gene Symbol

LRRFIP1

UniProt

NP_001131024.1 (Human)

Additional LRRFIP1 Products

Product Documents for LRRFIP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LRRFIP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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