Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S138-6

Concentration

1 mg/ml

View all LRRK2 Antibodies »

Product Summary for LRRK2 Antibody (S138-6)

Immunogen

Fusion protein, amino acids 1-500 (N-terminus) of human LRRK2. 83% identical in mouse and rat. No significant identity with LRRK1.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Mouse and Rat.

Localization

Cytoplasm , Membrane , Mitochondrion , Golgi Apparatus , Cell Projection , Axon , Dendrite , Endoplasmic Reticulum , Cytoplasmic Vesicle , Endosome , Lysosome

Specificity

Detects >200kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for LRRK2 Antibody (S138-6)

Western Blot: LRRK2 Antibody (S138-6) [NBP2-42175]

Western Blot: LRRK2 Antibody (S138-6) [NBP2-42175]

Western Blot: LRRK2 Antibody (S138-6) [NBP2-42175] - Western Blot analysis of Rat Brain Membrane showing detection of >200 kDa LRRK2/Dardarin protein using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S138-6 (NBP2-42175). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody (NBP2-42175) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: >200 kDa.
Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody (S138-6) [NBP2-42175]

Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody (S138-6) [NBP2-42175]

Immunocytochemistry/Immunofluorescence: LRRK2 Antibody (S138-6) [NBP2-42175] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S138-6 (NBP2-42175). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody (NBP2-42175) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LRRK2/Dardarin Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody (S138-6) [NBP2-42175]

Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody (S138-6) [NBP2-42175]

Immunocytochemistry/Immunofluorescence: LRRK2 Antibody (S138-6) [NBP2-42175] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Membrane, Nucleus. Magnification: 60X.

Applications for LRRK2 Antibody (S138-6)

Application
Recommended Usage

Western Blot

1:1000
Application Notes
1 ug/ml of LRRK2/Dardarin Antibody was sufficient for detection of LRRK2/Dardarin in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LRRK2

LRRK2 is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.

LRRK2 antibodies are useful tools for neurodegeneration research and certian kinase signaling pathway studies.

Long Name

Leucine-rich Repeat Ser/Thr Protein Kinase 2

Alternate Names

AURA17, Dardarin, PARK8, ROCO2

Gene Symbol

LRRK2

UniProt

Q5S007 (Human)

Additional LRRK2 Products

Product Documents for LRRK2 Antibody (S138-6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LRRK2 Antibody (S138-6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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