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Lubricin/PRG4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19048

Novus Biologicals, part of Bio-Techne
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NBP1-19048

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Synthetic peptide derived from N terminal domain of PRG4 protein

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23705804).

Specificity

Reacts specifically with human 151 kDa protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lubricin/PRG4 Antibody

Immunohistochemistry: Lubricin/PRG4 Antibody [NBP1-19048]

Immunohistochemistry: Lubricin/PRG4 Antibody [NBP1-19048]

Immunohistochemistry: Lubricin/PRG4 Antibody [NBP1-19048] - Sections of the tibial articular cartilage of 12-week-old normal Mig-6-flox (C) and Mig-6-flox; Prx1Cre conditional knockout (Mig-6-cko) knees (D) immunostained with antibodies against superficial zone protein (SZP, C, D). In normal articular cartilage, immunostaining is localized to cells mainly in the superficial zone (arrows in C) while in Mig-6-cko articular cartilage, immunostaining is dramatically increased and positive cells are located not only in the superficial zone but also in deeper zones (arrows in D). Scale Bar = 50 um. Image collected and cropped by CiteAb from the following publication (arthritis-research.biomedcentral.com/articles/10.1186/ar4233), licensed under a CC-BY license.
Immunohistochemistry: Lubricin/PRG4 Antibody [NBP1-19048]

Immunohistochemistry: Lubricin/PRG4 Antibody [NBP1-19048]

Immunohistochemistry: Lubricin/PRG4 Antibody [NBP1-19048] - Sections of six-week old tibial articular cartilage of normal Mig-6-flox (M) and Mig-6-cko (N) joints immunostained with antibodies against superficial zone protein (SZP, M, N). Enhanced marker expression by cells in the superficial and middle zones is evident in the six-week-old Mig-6-cko articular cartilage compared to control Mig-6-flox articular cartilage (arrows). Scale bar = 100 nm. Image collected and cropped by CiteAb from the following publication (arthritis-research.biomedcentral.com/articles/10.1186/ar4233), licensed under a CC-BY license.

Applications for Lubricin/PRG4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:200-1:1000
Application Notes
IHC-P, IHC reactivity reported in scientific literature (PMID: 23705804), (23083677).

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Lyophilized powder, reconstituted in distilled water.

Formulation

Antiserum

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lubricin/PRG4

Lubricin is a glycoprotein encoded by the PRG4 gene. It has been identified as a component of joint lubrication and synovial homeostasis by reducing friction in joints and preventing synovial cell overgrowth. It is a large, water soluble molecule that acts as a carrier for insoluble surface-active phospholipid (SAPL). Depletion of lubricin function has been associated with camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), an arthritic-like autosomal recessive disorder.

Long Name

Proteoglycan 4

Alternate Names

CACP, HAPO, Hemangiopoietin, JCAP, MSF, PRG4, Proteoglycan 4, SZP

Entrez Gene IDs

10216 (Human); 96875 (Mouse)

Gene Symbol

PRG4

UniProt

Additional Lubricin/PRG4 Products

Product Documents for Lubricin/PRG4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lubricin/PRG4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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