Lubricin/PRG4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19048
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Synthetic peptide derived from N terminal domain of PRG4 protein
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 23705804).
Specificity
Reacts specifically with human 151 kDa protein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Lubricin/PRG4 Antibody
Immunohistochemistry: Lubricin/PRG4 Antibody [NBP1-19048]
Immunohistochemistry: Lubricin/PRG4 Antibody [NBP1-19048] - Sections of the tibial articular cartilage of 12-week-old normal Mig-6-flox (C) and Mig-6-flox; Prx1Cre conditional knockout (Mig-6-cko) knees (D) immunostained with antibodies against superficial zone protein (SZP, C, D). In normal articular cartilage, immunostaining is localized to cells mainly in the superficial zone (arrows in C) while in Mig-6-cko articular cartilage, immunostaining is dramatically increased and positive cells are located not only in the superficial zone but also in deeper zones (arrows in D). Scale Bar = 50 um. Image collected and cropped by CiteAb from the following publication (arthritis-research.biomedcentral.com/articles/10.1186/ar4233), licensed under a CC-BY license.Immunohistochemistry: Lubricin/PRG4 Antibody [NBP1-19048]
Immunohistochemistry: Lubricin/PRG4 Antibody [NBP1-19048] - Sections of six-week old tibial articular cartilage of normal Mig-6-flox (M) and Mig-6-cko (N) joints immunostained with antibodies against superficial zone protein (SZP, M, N). Enhanced marker expression by cells in the superficial and middle zones is evident in the six-week-old Mig-6-cko articular cartilage compared to control Mig-6-flox articular cartilage (arrows). Scale bar = 100 nm. Image collected and cropped by CiteAb from the following publication (arthritis-research.biomedcentral.com/articles/10.1186/ar4233), licensed under a CC-BY license.Applications for Lubricin/PRG4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:200-1:1000
Application Notes
IHC-P, IHC reactivity reported in scientific literature (PMID: 23705804), (23083677).
Formulation, Preparation, and Storage
Purification
Unpurified
Reconstitution
Lyophilized powder, reconstituted in distilled water.
Formulation
Antiserum
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Lubricin/PRG4
Long Name
Proteoglycan 4
Alternate Names
CACP, HAPO, Hemangiopoietin, JCAP, MSF, PRG4, Proteoglycan 4, SZP
Gene Symbol
PRG4
UniProt
Additional Lubricin/PRG4 Products
Product Documents for Lubricin/PRG4 Antibody
Product Specific Notices for Lubricin/PRG4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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