Lunatic Fringe Antibody (JE66-62)

Western Blot: Lunatic Fringe Antibody (JE66-62) [NBP3-32530]

Western Blot: Lunatic Fringe Antibody (JE66-62) [NBP3-32530] - Western blot analysis of Lunatic Fringe on different lysates with Rabbit anti-Lunatic Fringe antibody (NBP3-32530) at 1/1,000 dilution.

Lane 1: Hela cell lysate (15 ug/Lane)
Lane 2: K-562 cell lysate (15 ug/Lane)
Lane 3: HL-60 cell lysate (15 ug/Lane)
Lane 4: MCF7 cell lysate (15 ug/Lane)
Lane 5: HT-29 cell lysate (15 ug/Lane)
Lane 6: C2C12 cell lysate (15 ug/Lane)
Lane 7: PC-12 cell lysate (15 ug/Lane)
Lane 8: Mouse testis tissue lysate (30 ug/Lane)

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 7 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32530) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: Lunatic Fringe Antibody (JE66-62) [NBP3-32530]

Immunocytochemistry/ Immunofluorescence: Lunatic Fringe Antibody (JE66-62) [NBP3-32530] - Immunocytochemistry analysis of HUVEC cells labeling Lunatic Fringe with Rabbit anti-Lunatic Fringe antibody (NBP3-32530) at 1/500 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lunatic Fringe antibody (NBP3-32530) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: Lunatic Fringe Antibody (JE66-62) [NBP3-32530]

Flow Cytometry: Lunatic Fringe Antibody (JE66-62) [NBP3-32530] - Flow cytometric analysis of K-562 cells labeling Lunatic Fringe.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32530, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).