LXR alpha/beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1465

Novus Biologicals, part of Bio-Techne
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NB100-1465
NB100-1465SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all LXR alpha/beta Antibodies »

Product Specifications

Immunogen

Peptide with sequence CRLQDKKLPPLLSEI corresponding to internal region according to NP_005684.2, NP_009052.3.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34564456). .

Specificity

This antibody is expected to recognise an epitope corresponding to aa 429-442 of human LXR alpha protein (NP_005684.2) and to aa 443-456 of human LXR beta protein (NP_009052.3).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LXR alpha/beta Antibody

Western Blot: LXR alpha/beta Antibody [NB100-1465]

Western Blot: LXR alpha/beta Antibody [NB100-1465]

Western Blot: LXR alpha/beta Antibody [NB100-1465] - Staining of Human Liver (A) and Kidney (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: LXR alpha/beta Antibody [NB100-1465]

Immunocytochemistry/ Immunofluorescence: LXR alpha/beta Antibody [NB100-1465]

Immunocytochemistry/Immunofluorescence: LXR alpha/beta Antibody [NB100-1465] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and weak cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: LXR alpha/beta Antibody [NB100-1465]

Flow Cytometry: LXR alpha/beta Antibody [NB100-1465]

Flow Cytometry: LXR alpha/beta Antibody [NB100-1465] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for LXR alpha/beta Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/mL
Application Notes
Western blot: Approx 55 kDa band observed in Human, Liver and Kidney lysates and in preliminary testing of Human Spleen lysate (calculated MW of 51.1 kDa according to NP_001238863.1). This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LXR alpha/beta

Liver X Receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of cholesterol metabolism and bile acids. Two known isoforms, alpha and beta, are differentially expressed. The alpha isoform is predominantly expressed in liver, whereas the beta isoform expression has been shown to be ubiquitous.

Alternate Names

LXRA, LXR-a, NR1H3 nuclear receptor subfamily 1, group H, member 3, RLD-1

Entrez Gene IDs

10062 (Human)

Gene Symbol

NR1H3

UniProt

NP_005684 (Human)

Additional LXR alpha/beta Products

Product Documents for LXR alpha/beta Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LXR alpha/beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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