Ly-6C Antibody (ER-MP20)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11242
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Immunoassay, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # ER-MP20
Concentration
0.1 mg/ml
Product Specifications
Immunogen
The monoclonal antibody ER-MP20 has been raised after immunization of rats with mouse macrophage cell lines and reacts with mouse macrophage precursor cells.
Epitope
The antigen is a 14 kD surface protein which is very similar to Ly-6C and may be analogous to human CD59.
Specificity
The monoclonal antibody ER-MP20 specifically reacts with mouse macrophage precursor cells in the mid-stage of their development (late CFU-M, monoblasts and monocytes). In tissue sections, the antigen is found on macrophage precursor subpopulations. ER-MP20 also detects a wide range of endothelial cells.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Ly-6C Antibody (ER-MP20)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunoassay
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Immunohistochemistry and Flow Cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.2 um filtered solution in PBS, 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Ly-6C
Alternate Names
Ly 6c, Ly6c protein, Lymphocyte antigen 6 complex, Lymphocyte antigen 6 complex locus C, Lymphocyte antigen 6C, Lymphocyte antigen Ly 6C
Gene Symbol
Ly6c1
Additional Ly-6C Products
Product Documents for Ly-6C Antibody (ER-MP20)
Product Specific Notices for Ly-6C Antibody (ER-MP20)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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