Ly-6C Antibody (PSH04-51)

Western Blot: Ly-6C Antibody (PSH04-51) [NBP3-32548]

Western Blot: Ly-6C Antibody (PSH04-51) [NBP3-32548] - Western blot analysis of Ly-6C on different lysates with Rabbit anti-Ly-6C antibody (NBP3-32548) at 1/1,000 dilution.

Lane 1: Mouse lung tissue lysate
Lane 2: Mouse testis tissue lysate (low expression)
Lane 3: Mouse liver tissue lysate (low expression)
Lane 4: Mouse kidney tissue lysate
Lane 5: Mouse heart tissue lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 14 kDa
Observed band size: 14 kDa

Exposure time: 3 minutes; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32548) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.