Ly-6E Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03570

Novus Biologicals, part of Bio-Techne
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NBP3-03570-100ul
NBP3-03570-20ul
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ly6E Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 21-101 of human Ly-6E (NP_002337.1). LMCFSCLNQKSNLYCLKPTICSDQDNYCVTVSASAGIGNLVTFGHSLSKTCSPACPIPEGVNVGVASMGISCCQSFLCNFS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ly-6E Antibody - BSA Free

Western Blot: Ly-6E AntibodyBSA Free [NBP3-03570]

Western Blot: Ly-6E AntibodyBSA Free [NBP3-03570]

Western Blot: Ly-6E Antibody [NBP3-03570] - Analysis of extracts of various cell lines, using Ly-6E antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Immunocytochemistry/ Immunofluorescence: Ly-6E Antibody - BSA Free [NBP3-03570]

Immunocytochemistry/ Immunofluorescence: Ly-6E Antibody - BSA Free [NBP3-03570]

Immunocytochemistry/Immunofluorescence: Ly-6E Antibody [NBP3-03570] - Analysis of L929 cells using LY6E Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. Ly-6E antibody
Immunohistochemistry-Paraffin: Ly-6E Antibody - BSA Free [NBP3-03570]

Immunohistochemistry-Paraffin: Ly-6E Antibody - BSA Free [NBP3-03570]

Immunohistochemistry-Paraffin: Ly-6E Antibody [NBP3-03570] - Left panel shows negative control antibody on mouse kidney; right panel shows Ly-6E antibody at 1:100 dilution on mouse kidney. Brown = DAB reaction. Mouse kidneys were deparaffinized through xylenes and alcohols, boiled in citrate buffer for 25 minutes, then PBS-Tween at room temp for 5 minutes, followed by Scytek CRF anti-polyvalent polymer DAB kit protocol. Slides were counterstained for 30 seconds in hematoxylin and coverslipped. Image from verified customer review.

Applications for Ly-6E Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

Validated from a verified customer review

Western Blot

1:500 - 1:1000

Reviewed Applications

Read 1 review rated 5 using NBP3-03570 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ly6E

Long Name

Lymphocyte Antigen 6 Family Member E

Alternate Names

Ly-6E, RIGE, SCA2, TSA1

Gene Symbol

LY6E

Additional Ly6E Products

Product Documents for Ly-6E Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ly-6E Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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