Ly-6H Antibody (3E10)
Novus Biologicals, part of Bio-Techne | Catalog # H00004062-M01
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rat
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
IF/IHC, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3E10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
LY6H (AAH28894, 26 a.a. ~ 140 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LWCQDCTLTTNSSHCTPKQCQPSDTVCASVRITDPSSSRKDHSVNKMCASSCDFVKRHFFSDYLMGFINSGILKVDVDCCEKDLCNGAAGAGHSPWALAGGLLLSLGPALLWAGP
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 23860910 and 25716842).
Localization
Cell membrane. Lipid-anchor, GPI-anchor.
Specificity
LY6H - lymphocyte antigen 6 complex, locus H
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Ly-6H Antibody (3E10)
Western Blot: Ly-6H Antibody (3E10) [H00004062-M01]
Western Blot: Ly-6H Antibody (3E10) [H00004062-M01] - Short-term nicotine exposure does not alter Lypd6, Lynx1, or Ly6H levels in the brain. Lypd6, Lynx1, Ly6H, and Beta2 nAChR subunit protein levels were analyzed in (a) frontal cortex (FC) and hippocampal (HIP) tissue from rats administered nicotine (0.4 mg/kg s.c., twice daily) or vehicle (0.9% saline) for 7 days from day 8-14 or (b) 54-60 (n = 8). (c and d) Representative images of western blots summarized in (a) and (b), respectively. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jnc.13718) licensed under a CC-BY license.Immunohistochemistry-Paraffin: Ly-6H Antibody (3E10) [H00004062-M01]
Immunohistochemistry-Paraffin: Ly-6H Antibody (3E10) [H00004062-M01] - Analysis of monoclonal antibody to LY6H on formalin-fixed paraffin-embedded human tonsil tissue. Antibody concentration 2 ug/ml.Western Blot: Ly-6H Antibody (3E10) [H00004062-M01]
Western Blot: Ly-6H Antibody (3E10) [H00004062-M01] - Ly-6H in mouse Ly-6H proteins using anti-Ly-6H antibody. Image from verified customer review.Applications for Ly-6H Antibody (3E10)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:500
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. It has been used for IHC-P and ELISA. Use in ICC reported in scientific literature (PMID: 25716842). Use in immunoprecipitation reported in scientific literature (PMID: 25716842). Use in Immunohistochemistry reported in scientific literature (PMID: 25716842).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using H00004062-M01 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
PBS (pH 7.4)
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Ly-6H
Alternate Names
Ly-6H, lymphocyte antigen 6 complex, locus H, lymphocyte antigen 6H, NMLY6
Entrez Gene IDs
4062 (Human)
Gene Symbol
LY6H
OMIM
603625 (Human)
UniProt
Additional Ly-6H Products
Product Documents for Ly-6H Antibody (3E10)
Product Specific Notices for Ly-6H Antibody (3E10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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