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LYPLA1 Antibody (3D5)

Novus Biologicals, part of Bio-Techne | Catalog # H00010434-M05

Novus Biologicals, part of Bio-Techne
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H00010434-M05

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3D5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

LYPLA1 (AAH08652.1, 66 a.a. ~ 151 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NVAMPSWFDIIGLSPDSQEDESGIKQAAENIKALIDQEVKNGIPSNRIILGGFSQGGALSLYTALTTQQKLAGVTALSCWLPLRAS

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:32927904)

Specificity

LYPLA1 - lysophospholipase I (3D5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for LYPLA1 Antibody (3D5)

Western Blot: LYPLA1 Antibody (3D5) [H00010434-M05]

Western Blot: LYPLA1 Antibody (3D5) [H00010434-M05]

Western Blot: LYPLA1 Antibody (3D5) [H00010434-M05] - Analysis of LYPLA1 expression in transfected 293T cell line by LYPLA1 monoclonal antibody (M05), clone 3D5. Lane 1: LYPLA1 transfected lysatE (24.7 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: LYPLA1 Antibody (3D5) [H00010434-M05]

Immunocytochemistry/ Immunofluorescence: LYPLA1 Antibody (3D5) [H00010434-M05]

Immunocytochemistry/Immunofluorescence: LYPLA1 Antibody (3D5) [H00010434-M05] - Analysis of monoclonal antibody to LYPLA1 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: LYPLA1 Antibody (3D5) [H00010434-M05] - Detection limit for recombinant GST tagged LYPLA1 is 0.1 ng/ml as a capture antibody.

Applications for LYPLA1 Antibody (3D5)

Application
Recommended Usage

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LYPLA1

Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine in both monomeric and micellar forms. The use of alternate polyadenylation sites has been found for this gene. There are alternatively spliced transcript variants described for this gene but the full length nature is not known yet. [provided by RefSeq]

Long Name

Acyl-protein thioesterase 1

Alternate Names

APT-1, EC 3.1.2.-, EC 3.1.2.22, hAPT1, LPL-I, LPL1, Lysophospholipase 1, Lysophospholipase I, LysoPLA I

Entrez Gene IDs

10434 (Human)

Gene Symbol

LYPLA1

Additional LYPLA1 Products

Product Documents for LYPLA1 Antibody (3D5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LYPLA1 Antibody (3D5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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