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LYPLAL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92090

Novus Biologicals, part of Bio-Techne
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NBP1-92090
NBP1-92090-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PMKGGISNVWFDRFKITNDCPEHLESIDVMCQVLTDLIDEEVKSGIKKNRILIGGFSMGGCMAMHLAYRNHQDVAGVFALSSF

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LYPLAL1 Antibody

Immunocytochemistry/ Immunofluorescence: LYPLAL1 Antibody [NBP1-92090]

Immunocytochemistry/ Immunofluorescence: LYPLAL1 Antibody [NBP1-92090]

Immunocytochemistry/Immunofluorescence: LYPLAL1 Antibody [NBP1-92090] - Staining of human cell line U-251 MG shows positivity in cytoplasm.
Immunohistochemistry-Paraffin: LYPLAL1 Antibody [NBP1-92090]

Immunohistochemistry-Paraffin: LYPLAL1 Antibody [NBP1-92090]

Immunohistochemistry-Paraffin: LYPLAL1 Antibody [NBP1-92090] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules and in glomeruli.
Simple Western: LYPLAL1 Antibody [NBP1-92090]

Simple Western: LYPLAL1 Antibody [NBP1-92090]

Simple Western: LYPLAL1 Antibody [NBP1-92090] - Lane view shows a specific band for LYPLA1 using 200 ug/mL of Sk-Mel-28 cell lysate and 1:4 antibody dilution. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 32 kDa. Image from an internal validation.

Applications for LYPLAL1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:20
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. See Simple Western Antibody Database for Simple Western validation: tested in SK-MEL-28; antibody dilution of 1:20; separated by size; detects a band at 32 kDa

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LYPLAL1

Long Name

Lysophospholipase-like protein 1

Alternate Names

EC 3.1.2.-, FLJ99730, KIAA1238, lysophospholipase-like 1, lysophospholipase-like protein 1, Q96AV0

Gene Symbol

LYPLAL1

Additional LYPLAL1 Products

Product Documents for LYPLAL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LYPLAL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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