Lys20p and Lys21p Antibody (31F5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05201

Novus Biologicals, part of Bio-Techne
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NBP1-05201
NBP1-05201-0.125ml
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Key Product Details

Species Reactivity

Yeast

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 31F5

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Lys20p and Lys21p Antibodies »

Product Specifications

Immunogen

Yeast nuclear preparation

Specificity

Lys20p and Lys21p

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

47.1/48.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lys20p and Lys21p Antibody (31F5)

Western Blot: Lys20p and Lys21p Antibody (31F5) [NBP1-05201]

Western Blot: Lys20p and Lys21p Antibody (31F5) [NBP1-05201]

Western Blot: Lys20p and Lys21p Antibody [NBP1-05201] - Western blots of whole yeast protein extracts with a collection of our antibodies. The blot for NBP1-05201 is in the indicated lane, and the number indicates the SDS-PAGE molecular weight in kiloDaltons.

Applications for Lys20p and Lys21p Antibody (31F5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000-1:10000

Immunohistochemistry

1:1000-1:10000

Western Blot

1:10000-1:50000
Application Notes
This Lys20p and Lys21p (31F5) antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In WB bands can be seen at 47.1 kDa (representing Lys20p) and 48.6 kDa (representing Lys21p).

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Supplied as concentrated hybridoma cell culture media.

Preservative

0.035% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lys20p and Lys21p

In the yeast Saccharomyces cerevisiae, AAA or alpha-amino adipate pathway is responsible for lysine biosynthesis wherein the first committed step involves condensation of acetyl CoA (AcCoA) with alpha-ketoglutarate (alpha-KG) to generate homocitrate as well as CoA in the nucleus, and this reaction is catalyzed by the homocitrate synthase isozymes encoded by LYS20 and LYS21 which belongs to alpha-IPM synthase/homocitrate synthase family and are 92% identical in amino acid sequence. Expression of both LYS20 and LYS21 is upregulated through Gcn4p upon amino acid deprivation, and their positive regulation is also facilitated by Lys14p (transcriptional activator from Zn-Cys cluster family) with co-inducer alpha-AASA (2-aminoadipate semialdehyde). Moreover, LYS20 and LYS21 are positively co-regulated by target of rapamycin or TOR signalling pathway coupled with protein kinase A, through a LYS14-GCN4-independent pathway. Therefore, LYS20 and LYS21 plays a key role in maintaining levels of lysine, an amino acid preferentially located in the vacuole, therefore not available as a source of nitrogen or carbon source, and lysine has been sown to play important physiological roles: contributing to the build-up of H2O2 triggered stress response, prevention of apoptosis induced upon amino acid starvation, and cryoprotection.

Alternate Names

LYS20 Lys20p

Additional Lys20p and Lys21p Products

Product Documents for Lys20p and Lys21p Antibody (31F5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lys20p and Lys21p Antibody (31F5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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