Lysine Hydroxylase 2/PLOD2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-58004

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Lysine Hydroxylase 2/PLOD2 Antibodies »

Product Summary for Lysine Hydroxylase 2/PLOD2 Antibody

Immunogen

Synthetic peptides corresponding to PLOD2(procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2) The peptide sequence was selected from the middle region of PLOD2. Peptide sequence IKGKTLRSEMNERNYFVRDKLDPDMALCRNAREMTLQREKDSPTPETFQM. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Lysine Hydroxylase 2/PLOD2 Antibody

Western Blot: Lysine Hydroxylase 2/PLOD2 Antibody [NBP1-58004]

Western Blot: Lysine Hydroxylase 2/PLOD2 Antibody [NBP1-58004]

Western Blot: Lysine Hydroxylase 2/PLOD2 Antibody [NBP1-58004] - Titration: 0.2-1 ug/ml, Positive Control: DU145 cell lysate.

Immunohistochemistry-Paraffin: Lysine Hydroxylase 2/PLOD2 Antibody [NBP1-58004] -

Immunohistochemistry-Paraffin: Lysine Hydroxylase 2/PLOD2 Antibody [NBP1-58004] - Formalin Fixed Paraffin; Embedded Tissue: Human Bronchial Epithelial Tissue; Observed Staining: Cytoplasmic; Primary Antibody Concentration: 1:100

Applications for Lysine Hydroxylase 2/PLOD2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine Hydroxylase 2/PLOD2

PLOD2 forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

LH2, PLOD2, TLH

Gene Symbol

PLOD2

UniProt

O00469-2 (Human)

Additional Lysine Hydroxylase 2/PLOD2 Products

Product Documents for Lysine Hydroxylase 2/PLOD2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Lysine Hydroxylase 2/PLOD2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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