Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (PSH01-77)

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (PSH01-77) [NBP3-32490]

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (PSH01-77) [NBP3-32490] - Western blot analysis of Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A on different lysates with Rabbit anti-Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A antibody (NBP3-32490) at 1/1,000 dilution.

Lane 1: HCT116 cell lysate
Lane 2: Hela cell lysate
Lane 3: MCF7 cell lysate
Lane 4: Ramos cell lysate
Lane 5: Jurkat cell lysate
Lane 6: NIH/3T3 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 147 kDa
Observed band size: 150 kDa

Exposure time: 2 minutes 50 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32490) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (PSH01-77) [NBP3-32490]

Immunohistochemistry: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (PSH01-77) [NBP3-32490] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A antibody (NBP3-32490) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32490) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.