Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49601

Novus Biologicals, part of Bio-Techne
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NBP1-49601
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.53 mg/ml

View all Lysine (K)-specific Demethylase 3A/KDM3A Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an internal region of the human JMJD1A protein (within residues 250-400). [Swiss-Prot Q9Y4C1]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27807143).

Localization

Nuclear in round spermatids. When spermatids start to elongate, JMJD1A localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A AntibodyBSA Free [NBP1-49601]

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A AntibodyBSA Free [NBP1-49601]

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-49601] - Analysis of JMJD1A in Cos7 CoCl2 treated lysate.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free [NBP1-49601]

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free [NBP1-49601]

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-49601] - Antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free [NBP1-49601]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free [NBP1-49601]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-49601] - Staining of JMJD1A in paraffin embedded mouse brain.
View 1 more images

Applications for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:2000

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:5000
Application Notes
This JMJD1A antibody is useful for immunohistochemistry on paraffin tissues, immunocytochemistry/immunofluorescence and Western blot where a band is seen ~147 kDa. In ICC/IF, nuclear signal was observed in MCF-7 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.53 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 3A/KDM3A

JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.

Alternate Names

JHDM2A, JMJD1A, KDM3A, KIAA0742, Lysine (K)specific Demethylase 3A, TSGA

Entrez Gene IDs

55818 (Human); 104263 (Mouse)

Gene Symbol

KDM3A

UniProt

Q9Y4C1, Q6PCM1 (Mouse)

Additional Lysine (K)-specific Demethylase 3A/KDM3A Products

Product Documents for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

Product Datasheets

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Product Specific Notices for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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