Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98966
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody
Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP2-98966]
Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP2-98966] - Anti-Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 147 kDa. Observed band size: 147 kDaImmunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP2-98966]
Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP2-98966] - Immunofluorescence staining of Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red).Positive staining was localized to cytoplasm and nucleus.Applications for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1500-1:50000
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Lysine (K)-specific Demethylase 3A/KDM3A
Alternate Names
JHDM2A, JMJD1A, KDM3A, KIAA0742, Lysine (K)specific Demethylase 3A, TSGA
Gene Symbol
KDM3A
Additional Lysine (K)-specific Demethylase 3A/KDM3A Products
Product Documents for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody
Product Specific Notices for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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