Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72553
![Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]](https://resources.bio-techne.com/images/products/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-OTI5B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72553-img0003.jpg "Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]")
![Western Blot: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9)Azide and BSA Free [NBP2-72553]](https://resources.bio-techne.com/images/products/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-OTI5B9-Azide-and-BSA-Free-Western-Blot-NBP2-72553-img0001.jpg "Western Blot: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9)Azide and BSA Free [NBP2-72553]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI5B9
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Summary
Immunogen
Full-length protein expressed in 293T cell transfected with human KDM4C expression vector
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images
Western Blot: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9)Azide and BSA Free [NBP2-72553]
Western Blot: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JMJD2C(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JMJD2C.![Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]](https://resources.bio-techne.com/images/products/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-OTI5B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72553-img0010.jpg "Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]")
Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]
Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553] - Staining of paraffin-embedded Ovary tissue using anti-JMJD2C mouse monoclonal antibody.![Flow Cytometry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]](https://resources.bio-techne.com/images/products/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-OTI5B9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72553-img0002.jpg "Flow Cytometry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]")
Flow Cytometry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]
Flow Cytometry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY JMJD2C.![Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]](https://resources.bio-techne.com/images/products/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-OTI5B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72553-img0004.jpg "Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]")
![Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]](https://resources.bio-techne.com/images/products/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-OTI5B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72553-img0005.jpg "Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]")
![Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]](https://resources.bio-techne.com/images/products/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-OTI5B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72553-img0006.jpg "Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]")
![Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]](https://resources.bio-techne.com/images/products/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-OTI5B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72553-img0007.jpg "Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]")
![Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]](https://resources.bio-techne.com/images/products/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-OTI5B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72553-img0008.jpg "Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]")
![Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]](https://resources.bio-techne.com/images/products/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-OTI5B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72553-img0009.jpg "Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]")
![Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]](https://resources.bio-techne.com/images/products/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-OTI5B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72553-img0011.jpg "Immunohistochemistry: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (OTI5B9) - Azide and BSA Free [NBP2-72553]")
Applications
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Lysine (K)-specific Demethylase 4C/KDM4C
Alternate Names
GASC1, JHDM3C, JMJD2C, KDM4C, Lysine (K)specific Demethylase 4C
Gene Symbol
KDM4C
Additional Lysine (K)-specific Demethylase 4C/KDM4C Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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