Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-18678]

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-18678] - Figure 2. Western blot analysis using the antibody directed against Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B , diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 179 kDa) is indicated on the right.

ELISA: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-18678]

ELISA: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-18678] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,200.