Lysozyme Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77790

Novus Biologicals, part of Bio-Techne
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NBP1-77790
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Key Product Details

Species Reactivity

Chicken

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Lysozyme Antibodies »

Product Specifications

Immunogen

This purified Lysozyme Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme. (Uniprot: Q6LEL2)

Reactivity Notes

A BLAST analysis was used to suggest that this antibody would react with all forms of lysozyme from chicken, including precursor as well as A and B chains. Chains designated as M, L, F, C, Y and D also show 100% sequence homology. Lysozyme from quail and pheasant are also reported to be 100% identical with HEW lysozyme. Cross reactivity against lysozyme from other sources may occur but has not been specifically determined

Specificity

This purified antibody is directed against lysozyme from hen egg white protein.

Marker

Histiocytic Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This purified antibody is directed against lysozyme from hen egg white protein. This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process, which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lysozyme (Hen Egg White)

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Lysozyme Antibody

Western Blot: Lysozyme Antibody [NBP1-77790]

Western Blot: Lysozyme Antibody [NBP1-77790]

Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane.Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C.Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT.(Right Blot-red)Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT.Block overnight at 4C.Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none.
Western Blot: Lysozyme Antibody [NBP1-77790]

Western Blot: Lysozyme Antibody [NBP1-77790]

Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody.Lane 1: Lysozyme (Hen Egg White).Load: 50 ng per lane.Primary antibody: Lysozyme antibody at 1:1,000 overnight at 4C.Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT.Block for 30 min at RT.Predicted/Observed size: 4.9 kDa, 4.9 kDa for Lysozyme (Hen Egg White).
ELISA: Lysozyme Antibody [NBP1-77790]

ELISA: Lysozyme Antibody [NBP1-77790]

ELISA: Lysozyme Antibody [NBP1-77790] - ELISA Results of Lysozyme antibody. Each well was coated in duplicate with 1.0 ug of Lysosome. The working dilution is 1:5000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.

Applications for Lysozyme Antibody

Application
Recommended Usage

ELISA

1:25000

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:100

Western Blot

1:500-1:2000
Application Notes
This product has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~14 kDa in size corresponding to lysozyme by western blotting in the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Lysozyme

Lysozyme catalyzes the hydrolysis of certain mucopolysaccharides of bacterial cell walls. Specifically, it catalyzes the hydrolysis of the bacterial cell wall beta glycosidic linkages between N acetylmuramic acid and N acetylglucosamine. It is found in spleen, lung, kidney, white blood cells, plasma, saliva, milk, and tears.

Alternate Names

EC 3.2.1.17, lysozyme, lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C, lysozyme C, LZM, renal amyloidosis

Gene Symbol

LYZ

UniProt

Q6LEL2 (Chicken)

Additional Lysozyme Products

Product Documents for Lysozyme Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Lysozyme Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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