Lysyl tRNA synthetase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1719
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Summary for Lysyl tRNA synthetase Antibody
Immunogen
The immunogen recognized by this antibody maps to a region between residue 550 and the C-terminus (residue 597) of human Lysyl-tRNA Synthetase using the numbering given in entry NP_005539.1 (GeneID 3735).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Lysyl tRNA synthetase Antibody
![Western Blot: Lysyl tRNA synthetase Antibody [NB100-1719]](https://resources.bio-techne.com/images/products/Lysyl-tRNA-synthetase-Antibody-Western-Blot-NB100-1719-img0002.jpg "Western Blot: Lysyl tRNA synthetase Antibody [NB100-1719]")
Western Blot: Lysyl tRNA synthetase Antibody [NB100-1719]
Western Blot: Lysyl tRNA synthetase Antibody [NB100-1719] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-Lysyl tRNA synthetase antibody NB100-1719 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).![Immunocytochemistry/ Immunofluorescence: Lysyl tRNA synthetase Antibody [NB100-1719]](https://resources.bio-techne.com/images/products/Lysyl-tRNA-synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1719-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Lysyl tRNA synthetase Antibody [NB100-1719]")
Immunocytochemistry/ Immunofluorescence: Lysyl tRNA synthetase Antibody [NB100-1719]
Immunocytochemistry/Immunofluorescence: Lysyl tRNA synthetase Antibody [NB100-1719] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Lysyl tRNA synthetase used at a dilution of 1:100.![Immunohistochemistry: Lysyl tRNA synthetase Antibody [NB100-1719]](https://resources.bio-techne.com/images/products/Lysyl-tRNA-synthetase-Antibody-Immunohistochemistry-NB100-1719-img0007.jpg "Immunohistochemistry: Lysyl tRNA synthetase Antibody [NB100-1719]")
Immunohistochemistry: Lysyl tRNA synthetase Antibody [NB100-1719]
Immunohistochemistry: Lysyl tRNA synthetase Antibody [NB100-1719] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:200 (1ug/ml). Detection: DABApplications for Lysyl tRNA synthetase Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 -1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1-4 ug/mg of lysate
Western Blot
1:2000-1:10000
Application Notes
Immunohistochemistry 1:100 to 1:500. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue section
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Lysyl tRNA synthetase
Alternate Names
CMTRIB, EC 6.1.1, EC 6.1.1.6, KARS2, KIAA0070, KRS, lysine tRNA ligase, Lysine--tRNA ligase, LysRS, lysyl-tRNA synthetase
Entrez Gene IDs
3735 (Human)
Gene Symbol
KARS1
UniProt
Additional Lysyl tRNA synthetase Products
Product Documents for Lysyl tRNA synthetase Antibody
Product Specific Notices for Lysyl tRNA synthetase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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