Lysyl tRNA synthetase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99362

Novus Biologicals, part of Bio-Techne
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NBP2-99362-100ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Lysyl tRNA synthetase Antibodies »

Product Summary for Lysyl tRNA synthetase Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Lysyl tRNA synthetase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Lysyl tRNA synthetase Antibody

Western Blot: Lysyl tRNA synthetase Antibody [NBP2-99362]

Western Blot: Lysyl tRNA synthetase Antibody [NBP2-99362]

Western Blot: Lysyl tRNA synthetase Antibody [NBP2-99362] - Anti-Lysyl tRNA synthetase rabbit polyclonal antibody at 1:500 dilution. Lane A: H1299 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 68 kDa. Observed band size: 68 kDa
Immunocytochemistry/ Immunofluorescence: Lysyl tRNA synthetase Antibody [NBP2-99362]

Immunocytochemistry/ Immunofluorescence: Lysyl tRNA synthetase Antibody [NBP2-99362]

Immunocytochemistry/Immunofluorescence: Lysyl tRNA synthetase Antibody [NBP2-99362] - Immunofluorescence staining of Lysyl tRNA synthetase in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Lysyl tRNA synthetase polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cytoplasm.
Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP2-99362]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP2-99362]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP2-99362] - Immunochemical staining of human Lysyl tRNA synthetase in human colon with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
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Applications for Lysyl tRNA synthetase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysyl tRNA synthetase

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis

Alternate Names

CMTRIB, EC 6.1.1, EC 6.1.1.6, KARS2, KIAA0070, KRS, lysine tRNA ligase, Lysine--tRNA ligase, LysRS, lysyl-tRNA synthetase

Gene Symbol

KARS1

Additional Lysyl tRNA synthetase Products

Product Documents for Lysyl tRNA synthetase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lysyl tRNA synthetase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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