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M-CSF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59425

Novus Biologicals, part of Bio-Techne
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NBP1-59425

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to MCSF The peptide sequence was selected from the middle region of MCSF. Peptide sequence SGSVLPLGELEGRRSTRDRRSPAEPEGGPASEGAARPLPRFNSVPLTDTG. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for M-CSF Antibody

Western Blot: M-CSF Antibody [NBP1-59425]

Western Blot: M-CSF Antibody [NBP1-59425]

Western Blot: M-CSF Antibody [NBP1-59425] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml
Immunohistochemistry-Paraffin: M-CSF Antibody [NBP1-59425]

Immunohistochemistry-Paraffin: M-CSF Antibody [NBP1-59425]

Immunohistochemistry-Paraffin: M-CSF Antibody [NBP1-59425] - Human Placenta Tissue, 10 ug/ml
Western Blot: M-CSF Antibody [NBP1-59425]

Western Blot: M-CSF Antibody [NBP1-59425]

Western Blot: M-CSF Antibody [NBP1-59425] - WB Suggested Anti-CSF1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: COLO205 cell lysate

Applications for M-CSF Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: M-CSF

Human Macrophage Colony Stimulating Factor (M-CSF, also called CSF-1) is a ~36 kDa disulfide-linked homodimer produced by a variety of cells including fibroblasts, macrophages, bone marrow stromal cells and endothelial cells. M-CSF regulates macrophage survival, proliferation and differentiation. M-CSF also enhances macrophage killing of tumor cells and microorganisms and regulates cytokine production from macrophages.

Long Name

Macrophage Colony Stimulating Factor

Alternate Names

CSF-1, CSF1, Lanimostim, MCSF

Gene Symbol

CSF1

UniProt

Additional M-CSF Products

Product Documents for M-CSF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for M-CSF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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