M-Ras/R-Ras3 Antibody (26E12)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26128
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2A Clone # 26E12
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for M-Ras/R-Ras3 Antibody (26E12)
Immunogen
Recombinant Human M-Ras/R-Ras3 protein [UniProt O14807]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for M-Ras/R-Ras3 Antibody (26E12)
Immunohistochemistry: M-Ras/R-Ras3 Antibody (26E12) [NBP3-26128] -
Immunohistochemistry: M-Ras/R-Ras3 Antibody (26E12) [NBP3-26128] - Image of M-Ras/R-Ras3 Antibody (26E12) diluted at 1:600 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Immunocytochemistry/Immunofluorescence: M-Ras/R-Ras3 Antibody (26E12) [NBP3-26128] -
Immunocytochemistry/Immunofluorescence: M-Ras/R-Ras3 Antibody (26E12) [NBP3-26128] - Staining of Hela cell with M-Ras/R-Ras3 Antibody (26E12) at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).Applications for M-Ras/R-Ras3 Antibody (26E12)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50% Glycerol, 0.01M PBS, PH 7.4
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: M-Ras/R-Ras3
Alternate Names
MRas, R-RAS3
Gene Symbol
MRAS
Additional M-Ras/R-Ras3 Products
Product Documents for M-Ras/R-Ras3 Antibody (26E12)
Product Specific Notices for M-Ras/R-Ras3 Antibody (26E12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...