Key Product Details

Species Reactivity

Virus

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # E1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all M13 bacteriophage Antibodies »

Product Specifications

Immunogen

Hybridoma produced by the fusion of splenocytes from mice immunized with isolated M13 phage coat proteins and mouse myeloma cells.

Reactivity Notes

M13 phage

Specificity

This antibody is specific for the M13 bacteriophage protein coat, amongst others the g3p protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Applications for M13 bacteriophage Antibody (E1)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100-1:2000
Application Notes
Base unit antibody (NBP2-66601) has been validated for use in Direct ELISA application, with protein coated on the well. We recommend a titration range starting with a 1:50 dilution for use in Direct ELISA..

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: M13 bacteriophage

Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage.May be used as a reagent in phage ELISA offering sensitive and specific activity for detection of recombinant phages.

Alternate Names

Coat protein A; Coat protein B; Coat protein C polypeptide I; Coat protein C polypeptide II; Coat protein D; G3P; III; IX; M13 coat protein A; M13 coat protein B; M13 coat protein C polypeptide I; M13 coat protein C polypeptide II; M13 coat protein D; M13

UniProt

P69168 (E. coli)

Additional M13 bacteriophage Products

Product Documents for M13 bacteriophage Antibody (E1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for M13 bacteriophage Antibody (E1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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