Macro H2A.2 Antibody (CL5263)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61156

Novus Biologicals, part of Bio-Techne
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NBP2-61156
NBP2-61156-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5263

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Macro H2A.2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a synthetic peptide corresponding to the following amino acid sequence:EKRGRKATSGKKGGK

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Macro H2A.2 Antibody (CL5263)

Immunocytochemistry/ Immunofluorescence: Macro H2A.2 Antibody (CL5263) [NBP2-61156]

Immunocytochemistry/ Immunofluorescence: Macro H2A.2 Antibody (CL5263) [NBP2-61156]

Immunocytochemistry/Immunofluorescence: Macro H2A.2 Antibody (CL5263) [NBP2-61156] - Staining of PC-3 cells using the Anti-H2AFY2 monoclonal antibody, showing specific staining in the nucleoplasm and nuclear membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry: Macro H2A.2 Antibody (CL5263) [NBP2-61156]

Immunohistochemistry: Macro H2A.2 Antibody (CL5263) [NBP2-61156]

Immunohistochemistry: Macro H2A.2 Antibody (CL5263) [NBP2-61156] - Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in renal glomeruli and a subset of renal tubules.
Immunohistochemistry: Macro H2A.2 Antibody (CL5263) [NBP2-61156]

Immunohistochemistry: Macro H2A.2 Antibody (CL5263) [NBP2-61156]

Immunohistochemistry: Macro H2A.2 Antibody (CL5263) [NBP2-61156] - Immunohistochemical staining of human testis shows strong nuclear immunoreactivity in a subset of cells in seminiferous tubules.
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Applications for Macro H2A.2 Antibody (CL5263)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Macro H2A.2

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation

Alternate Names

core histone macro-H2A.2, core histone macroH2A2.2, H2A histone family, member Y2, Histone macroH2A2, macroH2A2, mH2A2

Gene Symbol

MACROH2A2

Additional Macro H2A.2 Products

Product Documents for Macro H2A.2 Antibody (CL5263)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Macro H2A.2 Antibody (CL5263)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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