Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1C2

Concentration

1 mg/ml

View all Macro H2A.2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 2-261 of human H2AFY2 (NP_061119) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Macro H2A.2 Antibody (OTI1C2)

Western Blot: Macro H2A.2 Antibody (OTI1C2) [NBP2-45930]

Western Blot: Macro H2A.2 Antibody (OTI1C2) [NBP2-45930]

Western Blot: Macro H2A.2 Antibody (1C2) [NBP2-45930] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY H2A.2.
Immunohistochemistry: Macro H2A.2 Antibody (OTI1C2) [NBP2-45930]

Immunohistochemistry: Macro H2A.2 Antibody (OTI1C2) [NBP2-45930]

Immunohistochemistry: Macro H2A.2 Antibody (1C2) [NBP2-45930] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Macro H2A.2 Antibody (OTI1C2) [NBP2-45930]

Immunohistochemistry: Macro H2A.2 Antibody (OTI1C2) [NBP2-45930]

Immunohistochemistry: Macro H2A.2 Antibody (1C2) [NBP2-45930] - Analysis of Adenocarcinoma of Human breast tissue (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
View 17 more images

Applications for Macro H2A.2 Antibody (OTI1C2)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Macro H2A.2

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation

Alternate Names

core histone macro-H2A.2, core histone macroH2A2.2, H2A histone family, member Y2, Histone macroH2A2, macroH2A2, mH2A2

Gene Symbol

MACROH2A2

Additional Macro H2A.2 Products

Product Documents for Macro H2A.2 Antibody (OTI1C2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Macro H2A.2 Antibody (OTI1C2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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