MAD1L1/MAD1 Antibody (JE35-54)

Western Blot: MAD1L1/MAD1 Antibody (JE35-54) [NBP3-32556]

Western Blot: MAD1L1/MAD1 Antibody (JE35-54) [NBP3-32556] - Western blot analysis of MAD1L1/MAD1 on different lysates with Rabbit anti-MAD1L1/MAD1 antibody (NBP3-32556) at 1/1,000 dilution.

Lane 1: HCT 116 cell lysate
Lane 2: HeLa cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: Jurkat cell lysate
Lane 5: MCF7 cell lysate
Lane 6: A431 cell lysate
Lane 7: A375 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 83 kDa
Observed band size: 83 kDa

Exposure time: 15 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32556) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.