Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all MAD1L1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Mitotic arrest deficient-like 1 using the numbering given in Swiss-Prot entry Q9Y6D9 (GeneID 8379).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAD1L1/MAD1 Antibody

Western Blot: MAD1L1/MAD1 Antibody [NB100-569]

Western Blot: MAD1L1/MAD1 Antibody [NB100-569]

Western Blot: MAD1L1/MAD1 Antibody [NB100-569] - Detection of Human MAD1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAD1 antibody NB100-569 used for WB at 0.06 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NB100-569]

Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NB100-569]

Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NB100-569] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: MAD1L1/MAD1 Antibody [NB100-569]

Immunoprecipitation: MAD1L1/MAD1 Antibody [NB100-569]

Immunoprecipitation: MAD1L1/MAD1 Antibody [NB100-569] - Whole cell lysate (50 mcg/lane in A; 50 mcg input in B, 1 mg for IP in B) from HeLa cells. In A, HeLa cells were treated with Nocodazole (+) or mock treated (-). NB100-569 used at 0.1 mcg/ml for Western Blot (A) and at 1 mcg/mg lysate for IP (B). MAD1 was also immunoprecipitated using rabbit anti-MAD1 antibodies NB100-570 and NB100-571 using 1 mcg/mg lysate. In B, MAD1 in input and immunoprecipitate was detected using NB100-570 at 0.2 mcg/ml.

Applications for MAD1L1/MAD1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500- 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

1-4 ug/mg lysate

Western Blot

1:5000-1:15000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MAD1L1

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene.

Long Name

MAD1 Mitotic Arrest Deficient-Like 1

Alternate Names

HsMAD1, MAD1, PIG9, TP53I9, TXBP181

Entrez Gene IDs

8379 (Human)

Gene Symbol

MAD1L1

UniProt

Q9Y6D9

Additional MAD1L1 Products

Product Documents for MAD1L1/MAD1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAD1L1/MAD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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