MADD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06250

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MADD Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human MADD fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for MADD Antibody

Immunocytochemistry/ Immunofluorescence: MADD Antibody [NBP3-06250]

Immunocytochemistry/ Immunofluorescence: MADD Antibody [NBP3-06250]

Immunocytochemistry/Immunofluorescence: MADD Antibody [NBP3-06250] - Immunofluorescence staining of MADD in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MADD polyclonal antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Applications for MADD Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MADD

MAP kinase-activating death domain protein (MADD) is a death domain containing protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activate protein kinase (MAPK) and transduce apoptotic signals. MADD is a multi-pass membrane protein that exists as multiple alternatively spliced forms.

Alternate Names

DENNFLJ36300, Differentially expressed in normal and neoplastic cells, FLJ35600, IG20, Insulinoma glucagonoma clone 20, insulinoma-glucagonoma protein 20, KIAA0358RAB3GEP, MAP kinase-activating death domain protein, MAP-kinase activating death domain, Rab3 GDP/GTP exchange factor

Gene Symbol

MADD

Additional MADD Products

Product Documents for MADD Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MADD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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