MAG/Siglec-4a Antibody (PSH02-41)

Western Blot: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557]

Western Blot: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Western blot analysis of MAG/Siglec-4a on different lysates with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution.

Lane 1: Mouse brain(no heat) tissue lysate
Lane 2: Mouse cerebellum tissue lysate
Lane 3: Mouse liver tissue lysate (negative)
Lane 4: Rat brain(no heat) tissue lysate
Lane 5: Rat cerebellum tissue lysate
Lane 6: Rat liver tissue lysate (negative)

Lysates/proteins at 20 ug/Lane.

Predicted band size: 69 kDa
Observed band size: 100 kDa

Exposure time: 42 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32557) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557]

Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Immunofluorescence analysis of paraffin-embedded rat cerebellum tissue labeling MAG/Siglec-4a with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32557, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557]

Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32557) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.